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Title: Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
Authors: Ngan Thi Kim Pham
Quang Duy Trinh
Tuan Anh Nguyen
Shuvra Kanti Dey
Tung Gia Phan
Le Phuc Hoang
Pattara Khamrin
Niwat Maneekarn
Shoko Okitsu
Masashi Mizuguchi
Hiroshi Ushijima
Keywords: Immunology and Microbiology
Issue Date: 1-Mar-2009
Abstract: A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. © 2008 Elsevier B.V. All rights reserved.
ISSN: 01660934
Appears in Collections:CMUL: Journal Articles

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