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dc.contributor.authorNgan Thi Kim Phamen_US
dc.contributor.authorQuang Duy Trinhen_US
dc.contributor.authorTuan Anh Nguyenen_US
dc.contributor.authorShuvra Kanti Deyen_US
dc.contributor.authorTung Gia Phanen_US
dc.contributor.authorLe Phuc Hoangen_US
dc.contributor.authorPattara Khamrinen_US
dc.contributor.authorNiwat Maneekarnen_US
dc.contributor.authorShoko Okitsuen_US
dc.contributor.authorMasashi Mizuguchien_US
dc.contributor.authorHiroshi Ushijimaen_US
dc.description.abstractA nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. © 2008 Elsevier B.V. All rights reserved.en_US
dc.subjectImmunology and Microbiologyen_US
dc.titleDevelopment of genotype-specific primers for differentiation of genotypes A and B of Aichi virusesen_US
article.title.sourcetitleJournal of Virological Methodsen_US
article.volume156en_US of International Healthen_US's Hospital No. 1en_US Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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