Please use this identifier to cite or link to this item:
Title: Multiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patients
Authors: Aksara Thongprachum
Pattara Khamrin
Ngan Thi Kim Pham
Sayaka Takanashi
Shoko Okitsu
Hiroyuki Shimizu
Niwat Maneekarn
Satoshi Hayakawa
Hiroshi Ushijima
Keywords: Immunology and Microbiology
Issue Date: 1-May-2017
Abstract: © 2016 Wiley Periodicals, Inc. Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. J. Med. Virol. 89:818–824, 2017. © 2016 Wiley Periodicals, Inc.
ISSN: 10969071
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.