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dc.contributor.authorAksara Thongprachumen_US
dc.contributor.authorPattara Khamrinen_US
dc.contributor.authorNgan Thi Kim Phamen_US
dc.contributor.authorSayaka Takanashien_US
dc.contributor.authorShoko Okitsuen_US
dc.contributor.authorHiroyuki Shimizuen_US
dc.contributor.authorNiwat Maneekarnen_US
dc.contributor.authorSatoshi Hayakawaen_US
dc.contributor.authorHiroshi Ushijimaen_US
dc.description.abstract© 2016 Wiley Periodicals, Inc. Multiplex RT-PCR method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus A, B, and C, adenovirus, astrovirus, norovirus GI and GII, sapovirus, Aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and Saffold virus. The sensitivity of the method was evaluated and tested with 751 fecal specimens collected from Japanese children with acute diarrhea. Several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. Mixed-infection with different viruses in clinical specimens could also be effectively detected. The method proved to be reliable with highly sensitive and specific and useful for routine diagnosis. J. Med. Virol. 89:818–824, 2017. © 2016 Wiley Periodicals, Inc.en_US
dc.subjectImmunology and Microbiologyen_US
dc.titleMultiplex RT-PCR for rapid detection of viruses commonly causing diarrhea in pediatric patientsen_US
article.title.sourcetitleJournal of Medical Virologyen_US
article.volume89en_US University School of Medicineen_US of Tokyoen_US Mai Universityen_US Institute of Infectious Diseasesen_US
Appears in Collections:CMUL: Journal Articles

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