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Title: Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
Authors: Ingrid A. Beck
Rachel Payant
Nicole Ngo-Giang-Huong
Woottichai Khamduang
Laddawan Laomanit
Gonzague Jourdain
Lisa M. Frenkel
Keywords: Immunology and Microbiology
Issue Date: 1-Jul-2016
Abstract: © 2016 Elsevier B.V. Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (. OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings.
ISSN: 18790984
Appears in Collections:CMUL: Journal Articles

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