Effect of EDTA and Maleic acid on Microtensile bond Strength of Self-etch Adhesive Resin Cement after Temporary Cement Removal

Abstract

To investigate microtensile bond strength of self-etch adhesive resin cement to dentin after temporary cement removal by using EDTA and maleic acid. Forty-five extracted human molar were included in this study. Forty of the specimens were divided into two groups for temporary cementation on dentin using zinc oxide eugenol cement, and the others with zinc oxide non-eugenol cement. The remaining five specimens were used as a control group. After storing all specimens in distilled water at 37 oC for 24 hours, the specimens in the experimental groups were divided into 4 groups based on cleaning techniques: air water spray, pumice, 17% ethylenediaminetetraacetic acid (EDTA), and 10% maleic acid. To test microtensile bond strength, each specimen was bonded to a composite disc using self-etched adhesive resin cement before being sectioned into square rods with 1 mm2 cross sectional area. The microtensile bond strength was determined with a crosshead speed of 0.5 mm/minute. The data was statistically analyzed using two-way ANOVA with Holm-Sidak multiple comparison and one-way ANOVA on rank with Dunn's multiple comparison versus control (α=0.05). The fractured surfaces were examined using light microscope to classify the mode of failure. The use of EDTA on the zinc oxide non-eugenol group and maleic acid on the zinc oxide eugenol group resulted no significant difference on microtensile bond strength compared to control group. Removing zinc oxide non-eugenol cement using EDTA and removing zinc oxide eugenol cement using maleic acid provided microtensile bond strength comparable to those of uncontaminated dentin.