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Title: | A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing |
Authors: | Supakeit Chanarsa Jaroon Jakmunee Kontad Ounnunkad |
Authors: | Supakeit Chanarsa Jaroon Jakmunee Kontad Ounnunkad |
Keywords: | Chemistry |
Issue Date: | 28-Apr-2021 |
Abstract: | A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml−1 and 0.05 to 50 ng.ml−1, and the limit of detection (LOD) is estimated to be 0.86 pg.ml−1. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85105925491&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/76159 |
ISSN: | 22962646 |
Appears in Collections: | CMUL: Journal Articles |
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