Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/71153
Title: Antimicrobial effect of Japanese pumpkin (Cucurbita maxima) extract on local mastitis pathogen
Authors: Phacharaporn Tadee
Kridda Chukiatsiri
Doungporn Amornlerdpisan
Anon Paserakung
Nattinee Kittiwan
Authors: Phacharaporn Tadee
Kridda Chukiatsiri
Doungporn Amornlerdpisan
Anon Paserakung
Nattinee Kittiwan
Keywords: Japanese pumpkin;Polysaccharide;Carotenoids;Antimicrobial activity;Mastitis
Issue Date: 2020
Publisher: Faculty of Veterinary Medicine, Chiang Mai University
Citation: Veterinary Integrative Sciences (Vet Integr Sci) 18, 3 (Sep-Dec 2020), 141-152
Abstract: In Chiang Mai, Thailand, evidence of a high prevalence of bacterial mastitis with antimicrobial resistance has been observed over the past decade. The development of applicable alternative medicine options is needed to address this resistance situation. The crude extraction of natural resources, such as Japanese pumpkin, should be studied for bacterial activity so as to raise the economic value of each substance. The objective of this study was to explore the antimicrobial activity of Japanese pumpkin by-products against bacteria that has caused dairy mastitis to circulate in the Chiang Mai area. Crude polysaccharides and carotenoids were extracted from Japanese pumpkins. Subsequently, 55 isolates of 10 bacterial species were collected from mastitis milk samples obtained from dairy farms in Chiang Mai during August to October, 2018 were screened for antimicrobial activity using the disc diffusion method. Nine bacterial species displaying inhibition zones were then explored to determine the minimum inhibitory concentrations (MICs). Consequently, 300 mg/ml of crude polysaccharide extracts were not found to be able to inhibit most bacteria. However, the MICs of crude carotenoid extracts ranged from 75 to 300 mg/ml. In summary, by-products of the pumpkin extraction displayed effective antimicrobial activity. Thus, these by-products offer an alternative in preventing the bacteria causing mastitis. This indicates the possibility of developing a prototype of teat dip for the prevention of outbreaks of bacterial infections in the future among dairy cattle. Additionally, this approach could increase the potential value of a large amount of waste produced during the course of industrial processing.
Description: “Veterinary Integrative Sciences” is the official peer-reviewed journal of the Faculty of Veterinary Medicine, Chiang Mai University, Thailand. The primary aim of the journal is to facilitate and oversee the publication of a wide-range of high quality academic articles with an overall integration of the various areas of animal and veterinary sciences
URI: https://he02.tci-thaijo.org/index.php/vis/article/view/241209
http://cmuir.cmu.ac.th/jspui/handle/6653943832/71153
ISSN: 2629-9968
Appears in Collections:CMUL: Journal Articles

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