Please use this identifier to cite or link to this item:
Title: Accuracy of hemoglobin e screening test using allelic discrimination assay
Authors: Supranee Upanan
Somdet Srichairatanakool
Sirivipa Piyamongkol
Wirawit Piyamongkol
Keywords: Medicine
Issue Date: 30-Jun-2020
Abstract: © 2020 Royal Thai College of Obstetricians and Gynaecologists. All rights reserved. Objectives: To determine the accuracy of allelic discrimination (AD) assay for hemoglobin E (Hb E) screening test in Chiang Mai Strategy for thalassemia prevention and control. Materials and Methods: This study evaluated the AD assay compared with conventional Hb E screening tests used in Chiang Mai Strategy of Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand. In this assay, two TaqMan probes were designed to discriminate heterozygous and homozygous by detecting normal and mutant nucleotides of Hb E gene. Results: From 55 blinded DNA samples, the AD assay revealed the results with 100% sensitivity, specificity, positive predictive value, negative predictive value and efficiency when compared to the conventional Hb E screening tests of the Chiang Mai Strategy. Conclusion: The AD assay is effective as an Hb E screening test in the thalassaemia prevention and control program. Moreover, AD assay can distinguish heterozygous from homozygous genotypes.
ISSN: 26730871
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.