Mixed Micelle-mediated Cloud Point Extraction Coupled to Spectrophotometry for Fast Screening of Salbutamol in Wastewater, Pig Feed and Pork Samples

Abstract

A simple and rapid screening method for spectrophotometric determination of salbutamol based on mixed micelle-cloud point extraction (MM-CPE) coupled to derivatization with 4-aminoantipyrine reagent has been presented. A MM-CPE has been developed for the analysis of salbutamol in wastewater, pig feed and pork samples using Triton X-114 (TX-114) and cetyl trimethylammonium bromide (CTAB) as the mixed micellar extractant. The optimum MM-CPE conditions were: 10 mmol L-1 phosphate buffer pH 6.4, 5 mmol L-1 CTAB, 0.25% (w/v) TX-114, 10% (w/v) sodium chloride and 6 min equilibration time at 30 °C. And the optimum for derivatization were: 0.05 mol L-1 4-aminoantipyrine, 0.12 mol L-1 potassium hexacyanoferrate (III), 0.05 mol L-1 sodium carbonate and 1 min reaction time at room temperature. Under the selected condition, the proposed method gave linear calibrations in the range of 0.05 – 10 mg L-1. Limit of detection and limit of quantitation were 0.01 mg L-1 and 0.03 mg L-1, respectively. The proposed method has shown to be of high potential for analysis of salbutamol residues in wastewater, pig feed, and pork samples. The results of the proposed method agreed well with those obtained from HPLC (t-test, P=0.05).