Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/66579
Title: Determining effectiveness of rotavirus vaccine by immunochromatography and reverse transcriptase polymerase chain reaction: A comparison
Authors: Sheikh Ariful Hoque
Itoe Iizuka
Masaaki Kobayashi
Sayaka Takanashi
Kazi Selim Anwar
Mohammad Tajul Islam
Sk Azimul Hoque
Pattara Khamrin
Shoko Okitsu
Satoshi Hayakawa
Hiroshi Ushijima
Keywords: Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Veterinary
Issue Date: 16-Sep-2019
Abstract: © 2019 Elsevier Ltd Introduction: Because of the large animal reservoirs and reassortment capacity of rotaviruses (RVs) that pose the possibilities of waning the effectiveness of RV-vaccines, it remains essential to monitor vaccine effectiveness (VE) regularly. Although reverse transcription polymerase chain reaction (RT-PCR) remains sensitive for RV detection, physicians, especially in Japan, frequently use immunochromatography (IC)-based kits for RV diagnosis. Recently, IC is being used to calculate VE also. Herein, we investigated the validity of VEs determined by IC compared to that by RT-PCR during an outbreak in Shizuoka Prefecture, Japan. Methods: RVs in the stool or rectal swabs from children with acute gastroenteritis (AGE) were tested first by IC in the clinic and then by RT-PCR in the laboratory. A test-negative study design was used to examine VE. Results: Although the specificity of IC assay revealed 100%, its sensitivity remained weaker (67%) than that of RT-PCR that increased up to 88% depending on disease severity. VE assessed by IC remained stronger than that by RT-PCR: 79% (95% CI: 39–93%) by IC, and 58% (95% CI: −20% to 90%) by RT-PCR. However, VEs by IC and RT-PCR appeared almost similar in higher disease severity: 81.5% (95% CI: 40–94%) by IC and 72% (95% CI: 7–92%) by RT-PCR at severity ≥7, while 97.5% (95% CI: 77–99.7%) by IC and 92% (95% CI: 58–98%) by RT-PCR at severity ≥11. We showed that RV-vaccinated children had 80% [OR = 0.192 (95% CI: 0.052–0.709) less chance to be detected by IC. Conclusion: Although the sensitivity and specificity of IC differ by brand type, generally, IC is not as sensitive as RT-PCR. Despite the VEs remain higher by IC, it looks comparable with that of RT-PCR in severe cases implying that VEs evaluated by IC against severe illness remain useful for VE-monitoring.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85071077344&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/66579
ISSN: 18732518
0264410X
Appears in Collections:CMUL: Journal Articles

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