Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/65645
Title: Sperm can act as vectors for HIV-1 transmission into vaginal and cervical epithelial cells
Authors: Charlene D. Young
Suriya Tatieng
Kessiri Kongmanas
Duriya Fongmoon
Brett Lomenick
Alexander J. Yoon
Wongsakorn Kiattiburut
Federica Compostella
Kym F. Faull
Nuttee Suree
Jonathan B. Angel
Nongnuj Tanphaichitr
Keywords: Immunology and Microbiology
Medicine
Issue Date: 1-Jul-2019
Abstract: © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd Problem: Sperm are the major cells in semen. Human sperm possess a number of HIV-1 gp120 binding ligands including sulfogalactosylglycerolipid (SGG). However, the mechanisms of how sperm capture HIV-1 onto their surface are unclear. Furthermore, the ability of sperm to deliver HIV-1 to vaginal/cervical epithelial cells lining the lower female reproductive tract, as a first step in HIV-1 transmission, needs to be determined. Method of study: Sperm from healthy donors were incubated with dual-tropic HIV-1CS204 (clinical isolate), and virus capture was determined by p24 antigen ELISA. The involvement of SGG in HIV-1 capture was assessed by determining Kd values of HIV-1 gp120-SGG binding as well as computational docking of SGG to the gp120 V3 loop. The ability of sperm-associated HIV-1 to infect peripheral blood mononuclear cells (PBMCs) and TZM-bl indicator cells was determined. Lastly, infection of vaginal (Vk2/E6E7), ectocervical (Ect1/E6E7), and endocervical (End1/E6E7) epithelial cells mediated by HIV-1–associated sperm was evaluated. Results: Sperm were able to capture HIV-1 in a dose-dependent manner, and the capture reached a maximum within 5 minutes. Captured HIV-1, however, could be removed from sperm by Percoll-gradient centrifugation. Affinity of gp120 for SGG was substantial, implicating sperm SGG in HIV-1 capture. Sperm-associated HIV-1 could productively infect PBMCs and TZM-bl cells, and was capable of being transmitted into vaginal/cervical epithelial cells. Conclusion: Sperm are able to capture HIV-1, which remains infectious and is able to be transmitted into vaginal/cervical epithelial cells, a result indicating the importance of sperm in HIV transmission.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85067660122&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/65645
ISSN: 16000897
10467408
Appears in Collections:CMUL: Journal Articles

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