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Title: The chondroitin sulfate expression profi le in human amniotic fl uid cells: a time course study
Other Titles: การแสดงออกของสารคอนดรอยตินซัลเฟตในเซลล์น้ำคร่ำของมนุษย์ ในช่วงเวลาที่เพาะเลี้ยง
Authors: Sirinda Aungsuchawan
Junjira Keawdee
Chaniporn Puaninta
Nathaporn Pangjaidee
Siriwan Ong-Chai
Peraphan Pothacharoen
Issue Date: 2016
Publisher: Faculty of Medicine, Chiang Mai University
Abstract: Object The purpose of this study is to investigate the trend of expression of chondroitin sulfate epitope (WF6) in association with a continuous culture of hAFCs for a period of 30 days. Methods The hAFCs were obtained from the amniotic fl uid of pregnant women at 18 weeks of gestation (n=5). The cells were cultured in an RPMI 1,640 medium containing 20% FCS, Amnio- MAX-C100 16%, 0.03 mg/mL ampicillin and 0.1 mg/mL streptomycin (sigma, Buchs, Switzerland). The expression of chondroitin sulfate epitope (WF6) was detected continuously using immunocytochemistry and signal fl uorescence evaluated with the use of Image-pro Plus version 6.0. Results The levels of the mean area of the chondroitin sulfate WF6 was correlated with those of mean density. The fi rst cycle showed a gradual increase from day 0 to day 12 and then a continuous decrease from day 12 to day 18. In the second cycle the level increased from day 18 to day 27 and decreased after day 27 to day 30. Conclusion A reasonable conclusion is that the expression of chondroitin sulphate WF6 epitope in hAFCs gradually increased and then decreased in a cyclical pattern.
Description: Chiang Mai Medical Journal (Formerly Chiang Mai Medical Bulletin) is an official journal of the Faculty of Medicine, Chiang Mai University. It accepts original papers on clinical and experimental research that are pertinent in the biomedical sciences. The Journal is published 4 issues/year (i.e., Mar, Jun, Sep, and Dec).
ISSN: 0125-5983
Appears in Collections:CMUL: Journal Articles

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