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Title: Red jasmine rice extract suppresses genes associated with cartilage degradation in IL-1β-stimulated chondrosarcoma (SW1353) via blocking NF-κB pathway
Other Titles: สารสกัดข้าวหอมมะลิแดงยับยั้งยีนซึ่งเกี่ยวข้องกับการสลายกระดูกอ่อนที่กระตุ้นโดย อินเตอร์ลิวคิน-1บีตา ผ่านการยับยั้งวิถี NF-κB ในเซลล์ SW1353 เพาะเลี้ยง
Authors: Supitcha Thonghoi
Rungnaree Jaitham
Patiwat Kongdang
Supachai Yodkeeree
Siriwan Ongchai
Pornngarm Limtrakul
Issue Date: 2018
Publisher: Faculty of Medicine, Chiang Mai University
Abstract: Objective This study aimed to evaluate the chondroprotective potential of red jasmine rice crude extract (RRE) against an expression of genes associated with cartilage degradation stimulated by IL-1β in a human chondrosarcoma cell line, SW1353, culture model. Methods SW1353 cells were pre-incubated with 1 ng/mL of recombinant human interleukin-1β (IL-1β) followed by the addition of RRE in a range of concentrations from of 0 to 100 μg/mL. After 24 hours of incubation, cells were harvested and expression of genes associated with cartilage degradation was measured using real-time RT-PCR. The culture media were evaluated for MMP-13 levels using an ELISA kit. Upregulation of the signaling pathway which promotes the infl ammatory cascade, nuclear factor kappa B (NF-κB), was determined by Western blot analysis. Results Cytokine IL-1β signifi cantly upregulated the expression of the proinfl ammatory mediators IL-1β, IL-6, TNF-α, and COX-2 as well as enzymes involved in osteoarthritis such as MMP-13, ADAMTS-4, and ZIP-8. The results showed that RRE at a concentration of 100 μg/mL signifi cantly suppressed IL-1β-induced expression of these genes. Moreover, the effect of IL-1β on NF-κB signaling pathway activation was suppressed by RRE via decreased phosphorylated form of IKKα/β, IκBα, and NF-κB p65. Conclusion RRE has chondroprotective potential through reduction of the gene upregulating effect of IL-1β which involve cartilage degradation via suppression of the NF-κB signaling pathway. Development of RRE has for alternative use potential in treating degenerative joint diseases.
Description: Chiang Mai Medical Journal (Formerly Chiang Mai Medical Bulletin) is an official journal of the Faculty of Medicine, Chiang Mai University. It accepts original papers on clinical and experimental research that are pertinent in the biomedical sciences. The Journal is published 4 issues/year (i.e., Mar, Jun, Sep, and Dec).
ISSN: 0125-5983
Appears in Collections:CMUL: Journal Articles

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