Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/62421
Title: Laboratory investigations of Hb Constant Spring
Authors: Orathai Tangvarasittichai
R. Jeenapongsa
C. Sitthiworanan
T. Sanguansermsri
Keywords: Medicine
Issue Date: 1-Feb-2005
Abstract: Hb Constant Spring (HbCS), a nondeletional α-thalassemia, is most prevalent in southern Chinese and southeast Asian populations. In conjunction with α-thalassemia-1 or in the homozygous state, it is an important cause of HbH disease. The present study was designed to test the efficiency of different diagnostic methods in detecting HbCS. The following laboratory tests were applied to blood samples from 1000 pregnant women attending an antenatal clinic at Buddhachinaraj Hospital in Phitsanulok, Thailand: mutation specific restriction enzyme digestion (RED), amplification refractory mutation system (ARMS) and automated high-performance liquid chromatography (HPLC). The results demonstrate that the DNA-based methods, RED and ARMS, are efficient diagnostic tools in detecting HbCS in homozygotes and heterozygotes, whereas automated HPLC gave accurate results for homozygous HbCS, but misidentified (no peak) some cases of HbCS trait. Standard hematological methods (determination of mean cell hemoglobin and mean cell volume) did not efficiently differentiate homozygous HbCS and HbCS trait from samples with normal α-globin chains. We conclude that RED is the preferable method for HbCS screening and then confirmed diagnostic testing using ARMS. Cases unresolved by ARMS should be clarified by appropriate methods such as DNA sequencing. © 2005 Blackwell Publishing Ltd.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=14344255483&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/62421
ISSN: 01419854
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.


Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.