Please use this identifier to cite or link to this item:
Title: Biotransformation of cortexolone to hydrocortisone by molds using a rapid color-development assay
Authors: J. Manosroi
Y. Chisti
A. Manosroi
Keywords: Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Issue Date: 1-Sep-2006
Abstract: The capacity of 22 molds for 11β-hydroxylation of cortexolone (Reichstein's compound S) to hydrocortisone were assessed. The biotransformation capacity was compared for solid-state and submerged monocultures of molds that were otherwise under identical conditions. Thin-layer chromatography and a novel rapid color-development assay were used to qualitatively establish the ability of fungi to convert cortexolone to hydrocortisone. These assays were validated and supplemented with data from high-performance liquid chromatography to obtain quantitative information on biotransformation. Nearly all the fungi consumed a significant fraction of the cortexolone fed, but only four of them (i.e., two isolates of Cunninghamella blakesleeana, C. echinulata, and Curvularia lunata) yielded measurable quantities of hydrocortisone. Submerged cultures generally gave a significantly greater yield of hydrocortisone compared to equivalent solid-state cultures. © MAIK "Nauka/Interperiodica" (Russia), 2006.
ISSN: 00036838
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.