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Title: High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
Authors: Pralongyut Sripalwit
Chalobol Wongsawad
Pheravut Wongsawad
Somboon Anuntalabhochai
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2007
Abstract: The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers. © 2006 Elsevier Inc. All rights reserved.
ISSN: 10902449
Appears in Collections:CMUL: Journal Articles

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