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Title: Role of chitin binding domain of chitinase A of streptomyces cyaneus SP-27 in protoplast formation from schizophyllum commune
Authors: Shigekazu Yano
Arata Honda
Nopakarn Rattanakit-Chandet
Noda Yuta
Mamoru Wakayama
Abhinya Plikomol
Takashi Tachiki
Keywords: Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Issue Date: 30-Apr-2009
Abstract: Chitinase A (CHIA) of Streptomyces cyaneus SP-27 forms protoplasts from Schizophyllum commune mycelia when it is combined with α-1,3-glucanase of Bacillus circulans KA-304. An N-terminal chitin-binding domain truncated mutant (CatCHIA), which was expressed in Escherichia coli Rosetta-gami B (DE 3), lost most of its colloidal chitin- and powder chitin-binding activity. The colloidal chitin-hydrolyzing, the powder chitinhydrolyzing, and the protoplast-forming activities of CatCHIA were lower than those of CHIA, suggesting that the chitin-binding domain contributes to the hydrolysis of chitin in the cell-wall of S. commune.
ISSN: 13476947
Appears in Collections:CMUL: Journal Articles

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