Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/58220
Title: A sensitive electrochemical immunosensor based on poly(2-aminobenzylamine) film modified screen-printed carbon electrode for label-free detection of human immunoglobulin G
Authors: Thitirat Putnin
Watthanachai Jumpathong
Rawiwan Laocharoensuk
Jaroon Jakmunee
Kontad Ounnunkad
Keywords: Biochemistry, Genetics and Molecular Biology
Engineering
Medicine
Pharmacology, Toxicology and Pharmaceutics
Issue Date: 4-Jul-2018
Abstract: © 2017 Informa UK Limited, trading as Taylor & Francis Group. This work focuses on fabricating poly(2-aminobenzylamine)-modified screen-printed carbon electrode as an electrochemical immunosensor for the label-free detection of human immunoglobulin G. To selectively detect immunoglobulin G, the anti-immunoglobulin G antibody with high affinity to immunoglobulin G was covalently linked with the amine group of poly(2-aminobenzylamine) film-deposited screen-printed carbon electrode. The selectivity for immunoglobulin G was subsequently assured by being challenged with redox-active interferences and adventitious adsorption did not significantly interfere the analyte signal. To obviate the use of costly secondary antibody, the [Fe(CN)6]4-/3-redox probe was instead applied to measure the number of human immunoglobulin G through the immunocomplex formation that is quantitatively related to the level of the differential pulse voltammetric current. The resulting immunosensor exhibited good sensitivity with the detection limit of 0.15 ng mL−1, limit of quantitation of 0.50 ng mL−1and the linear range from 1.0 to 50 ng mL−1. Given those striking analytical performances and the affordability arising from using cheap screen-printed carbon electrode with label-free detection, the immunosensor serves as a promising model for the next-step development of a diagnostic tool.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026920440&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/58220
ISSN: 2169141X
21691401
Appears in Collections:CMUL: Journal Articles

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