Please use this identifier to cite or link to this item:
Title: Evaluation of immunochromatographic tests and a new enzyme immunoassay for detection of a novel GII.17 norovirus in stool samples
Authors: Hiroshi Ushijima
Aksara Thongprachum
Pattara Khamrin
Sayaka Takanashi
Shoko Okitsu
Niwat Maneekarn
Satoshi Hayakawa
Keywords: Medicine
Issue Date: 1-Jan-2017
Abstract: © 2017, National Institute of Health. All rights reserved. A novel GII.17 norovirus (NoV), Kawasaki 2014, has spread to several regions of the world. Rapid and reliable diagnostic tests are needed for the detection of this new NoV variant. In this study,analytical sensitivity of 7 different immunochromatographic (IC) test kits (6 are on the market in Japan and one in Europe) was evaluated by means of confirmed GII.17 NoV-positive stool samples. The stool samples were also tested by a bioluminescent enzyme immunoassay (BLEIA). Real-time RT-PCR served as a reference (gold standard) method. Among the 7 IC kits, RIDA QUICK was the most sensitive, with the limit of detection of 107copies/ml, whereas the limits of detection of the other IC kits ranged from 108to 109copies/ml. It should be pointed out that the limit of detection of BLEIA was approximately 100- to 1,000-fold better (104–105copies/ml) than that of RIDA QUICK. Nevertheless, the procedure of BLEIA took more time and required sophisticated equipment.
ISSN: 18842836
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.