Please use this identifier to cite or link to this item:
Title: Simultaneous flow cytometric measurement of antigen attachment to phagocytes and phagocytosis
Authors: Witida Laopajon
Nuchjira Takheaw
Watchara Kasinrerk
Supansa Pata
Keywords: Biochemistry, Genetics and Molecular Biology
Health Professions
Immunology and Microbiology
Issue Date: 2-Sep-2016
Abstract: © 2016 Taylor & Francis. The current available assays cannot differentiate the stages of phagocytosis. We, therefore, established methods for concurrent detection of antigen attachment and engulfment by phagocyte using latex beads coated with lipopolysaccharide, rabbit IgG, and carboxyfluorescein diacetate succinimidyl ester. The generated beads were incubated with whole blood at 37°C for 1 hr and stained with PE-Cy5.5 anti-rabbit IgG antibody. By flow cytometry, attachment and phagocytic processes could be detected, simultaneously. The established method is a valuable tool for diagnosis of phagocytic disorder and study of molecules involved in phagocytosis.
ISSN: 15324230
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.