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Title: Effects of chitosan and salicylic acid on stemona alkaloid production in hydroponic culture of stemona curtisii Hook. f.
Authors: Jiraporn Palee
Srisulak Dheeranupattana
Sunanta Wangkarn
Stephen G. Pyne
Alison T. Ung
Keywords: Biochemistry, Genetics and Molecular Biology
Materials Science
Physics and Astronomy
Issue Date: 1-Oct-2016
Abstract: © 2016, Chiang Mai University. All rights reserved. The objective of this study was to investigate the effects of the elicitors, salicylic acid (SA) and chitosan, on the improvement of Stemona alkaloid production in hydroponic cultures of S. curtisii. In vitro plantlets were used as plant materials. The elicitors were added into the culture medium and samples of the roots and medium were collected on week 2 and 4 after the elicitor addition and then analyzed for Stemona alkaloid production by high performance liquid chromatography (HPLC). This study revealed that both SA and chitosan increased production of three Stemona alkaloids and that chitosan is better than SA for the enhancement of the production of these alkaloids. The elicitation by 20 mg L-1of chitosan for 4 weeks induced highest amount of oxyprotostemonine (274.31 μg g-1DW) stemocurtisine (35.46 μg g-1DW) and stemocurtisinol (99.48μg g-1DW), which were 1.9, 2.0 and 1.5 fold higher than that of the control, respectively.
ISSN: 01252526
Appears in Collections:CMUL: Journal Articles

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