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Title: Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease
Authors: Kanet Pakdeepak
Supansa Pata
Sawitree Chiampanichayakul
Watchara Kasinrerk
Thanusak Tatu
Keywords: Biochemistry, Genetics and Molecular Biology
Health Professions
Immunology and Microbiology
Issue Date: 1-Nov-2016
Abstract: © 2016 Taylor & Francis. Monoclonal antibodies against α-globin containing human Hbs, named AMS-Alpha1 and AMS-Alpha 2, were produced by the hybridoma technique using spleen cells enriched by the newly developed B lymphocyte enrichment protocol. These two monoclonal antibodies were of IgM class, reacting to only intact form of human Hbs A, A2, E, and F, which contain α-globin chain. By the indirect ELISA, the AMS-Alpha1 and AMS-Alpha 2 quantified less amount of α-globin chain containing hemoglobins in HbH disease than the SEA-α thalassemia 1 carriers and normal individuals. It was thus anticipated that these monoclonal antibodies can be used for detecting Hb Bart’s hydrops fetalis in which no α-globin chain is produced.
ISSN: 15324230
Appears in Collections:CMUL: Journal Articles

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