Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/53035
Title: Genomic DNA isolation from dried blood using gelatin-coated magnetic particles
Authors: Sorasak Intorasoot
Jintara Techateerawat
Amornrat Intorasoot
Keywords: Multidisciplinary
Issue Date: 1-Jul-2013
Abstract: Gelatin-coated magnetic particles were implemented for isolation of human genomic DNA from dried blood spotted onto glass microfibre filters. The DNA quality and quantity were compared to a silica-based membrane purification kit. Conditions for dried blood storage were varied for DNA stability determination. Approximately three times higher DNA yield was recovered by coated magnetic particles than the silicabased kit. In addition, the quality of extracted DNA was determined using β-actin gene amplification by polymerase chain reaction (PCR). It was implied that genomic DNA was stable for at least 28 days in a dried condition. Gelatin-coated magnetic particles are rapid, simple and useful for DNA extraction in field applications.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880650274&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/53035
ISSN: 00113891
Appears in Collections:CMUL: Journal Articles

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