Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/52622
Title: Rapid detection and identification of Wuchereria bancrofti, Brugia malayi, b. pahangi, and Dirofilaria immitis in mosquito vectors and blood samples by high resolution melting real-time PCR
Authors: Tongjit Thanchomnang
Pewpan M. Intapan
Chairat Tantrawatpan
Viraphong Lulitanond
Sudchit Chungpivat
Piyanan Taweethavonsawat
Worasak Kaewkong
Oranuch Sanpool
Penchom Janwan
Wej Choochote
Wanchai Maleewong
Keywords: Immunology and Microbiology
Medicine
Issue Date: 1-Dec-2013
Abstract: A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2°C, 79.0±0.3°C, 76.8±0.1°C, and 79.9±0.1°C, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors. © 2013, Korean Society for Parasitology and Tropical Medicine.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84893315121&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52622
ISSN: 17380006
00234001
Appears in Collections:CMUL: Journal Articles

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