Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/52237
Title: Micellar liquid chromatographic determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products
Authors: W. Thogchai
B. Liawruangrath
Keywords: Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Chemistry
Medicine
Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jun-2013
Abstract: A simple micellar liquid chromatographic (MLC) procedure for simultaneous determination of arbutin and hydroquinone in medicinal plant extracts and commercial cosmetic products was proposed. This method was developed and validated. The chromatographic conditions were also optimized. All analyses were performed at room temperature in an isocratic mode, using a mixture of 1% (v/v) acetonitrile and 0.006 mol L-1 Brij 35 (pH 6.0) as a mobile phase. The flow rate was set at 1.0 mL min-1. The analytical column was a 150 × 3.9 mm Nova-Pak C-18 column. The effluent from the analytical column was monitored by UV detection at 280 nm. Under the optimum conditions, arbutin and hydroquinone could be determined within a concentration range of 2-50 μg mL-1 of arbutin, and hydroquinone was obtained with the regression equations; y = 0.045x + 0.042 (r2 = 0.9923) and y = 0.091x + 0.050 (r2 = 0.9930) respectively. The limits of detection were found to be 0.51 μg mL-1 and 0.37 μg mL-1 for arbutin and hydroquinone respectively. The proposed MLC method was applied for the determination of arbutin and hydroquinone contents in medicinal plant extracts and commercial cosmetic products. This proposed MLC method is thus suitable for routine analysis of arbutin and hydroquinone in the pharmaceutical formulations, cosmetic products and raw medicinal plant extracts. © ICS © 2013 Society of Cosmetic Scientists and the Société Française de Cosmétologie.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84877827753&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/52237
ISSN: 14682494
01425463
Appears in Collections:CMUL: Journal Articles

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