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|Title:||Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.|
|Keywords:||Biochemistry, Genetics and Molecular Biology|
Physics and Astronomy
|Abstract:||In vitro propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L-1benzyladenine (BA) to produce multiple shoots. This research revealed that the medium containing 2 mg L-1BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 μg g-1DW, stemocurtisine 7 μg g-1DW and stemocurtisinol 9 μg g-1DW.|
|Appears in Collections:||CMUL: Journal Articles|
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