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Title: Effects of BA and NAA on micropropagation and Stemona alkaloids production of Stemona curtisii Hook.f.
Authors: Jiraporn Palee
Srisulak Dheeranupattana
Araya Jatisatienr
Sunanta Wangkarn
Keywords: Biochemistry, Genetics and Molecular Biology
Materials Science
Physics and Astronomy
Issue Date: 6-Aug-2013
Abstract: In vitro propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L-1benzyladenine (BA) to produce multiple shoots. This research revealed that the medium containing 2 mg L-1BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 μg g-1DW, stemocurtisine 7 μg g-1DW and stemocurtisinol 9 μg g-1DW.
ISSN: 01252526
Appears in Collections:CMUL: Journal Articles

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