Effects of sodium acetate and sucrose on in vitro alkaloid production from Stemona sp. culture

Abstract

Precursor feeding through plant tissue culture is provided to enhance plant secondary metabolite production. The effects of sodium acetate and sucrose on Stemona alkaloids were investigated. Shoot tips and auxiliary buds of Stemona sp. were cultured on MS agar medium supplemented with 3 mg L-1 benzylademne for multiple shoot induction. Single shoots were then transferred to half-MS medium supplemented with 2 mg L-1 indolebutyric acid for root induction. Stemona plantlets were cultured on various concentrations of sodium acetate and sucrose and the alkaloids extract were determined. It was shown that both of them could enhance Stemona alkaloids production. The most effective precursor was 25 mg L-1 sodium acetate which gave the highest production of 1′,2′-didehydrostemofoline and stemofoline at 2.35 and 2.04 folds higher than the control cultures, respectively. The study concluded that sodium acetate was the appropriate precursor than sucrose for enhancing Stemona alkaloids production. © 2013 Asian Network for Scientific Information.