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Title: DNA barcoding for the identification of eight species members of the Thai Hyrcanus Group and investigation of their stenogamous behavior
Authors: Adulsak Wijit
Atiporn Saeung
Visut Baimai
Yasushi Otsuka
Sorawat Thongsahuan
Kritsana Taai
Wichai Srisuka
Siripan Songsawatkiat
Sriwatapron Sor-Suwan
Chayanit Hempolchom
Pradya Somboon
Wej Choochote
Keywords: Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Issue Date: 1-Sep-2013
Abstract: Eight species members of the Thai Hyrcanus Group were identified based on the intact morphology and molecular analysis (COI barcoding, 658 bp) of F1-progenies. Five iso-female lines of each species were pooled in order to establish stock colonies. A stenogamous colony of each species was investigated by making 200 and 300 newly emerged adult females and males co-habit in a 30 cm cubic cage for one week. After ovipositon, the spermathecae of females were examined for sperms. The results revealed that Anopheles argyropus, Anopheles crawfordi, Anopheles nitidus, Anopheles pursati, Anopheles sinensis, Anopheles nigerrimus, Anopheles paraliae and Anopheles peditaeniatus yielded insemination rates of 0%, 0%, 0%, 31%, 33%, 42%, 50% and 77%, respectively. Continuous selection to establish stenogamous colonies indicated that An. sinensis, An. pursati, An. nigerrimus, An. paraliae and An. peditaeniatus provided insemination rates of 33-34%, 27-31%, 42-58%, 43-57% and 61-86% in 1, 2, 5, 6 and 20 generations of passages, respectively. © 2013 Académie des sciences.
ISSN: 17683238
Appears in Collections:CMUL: Journal Articles

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