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Title: Evaluation and comparison of the efficiency of immunochromatography methods for norovirus detection
Authors: Aksara Thongprachum
Pattara Khamrin
Dinh Nguyen Tran
Shoko Okitsu
Masashi Mizuguchi
Satoshi Hayakawa
Niwat Maneekarn
Hiroshi Ushijima
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 16-Jul-2012
Abstract: Background: The efficiency of a commercial immunochromatography kit (IP-NoV) was evaluated for rapid detection of norovirus in Japanese fecal specimens and its sensitivity and specificity were compared with two other commercial kits. Methods: A total of 70 samples collected from children who suffered from acute gastroenteritis in 2009-2010 were tested for norovirus by three different immunochromatography kits. RT-PCR was employed as the gold standard method. Results: The sensitivity of IP-NoV, QuickEx-Norovirus, and QuickNavi-Norovirus kits were 85.2%, 63.9%, and 55.7%, respectively. The IP-NoV kit could detect various genotypes of norovirus with higher efficiency as compared to QuickEx-Norovirus and QuickNavi-Norovirus kits. In addition, the phylogenetic analysis revealed that the majority of noroviruses circulating in Japan during 2009-2010 belonged to GII/4 variant 2006b and 2008a, which were responsible for the outbreaks worldwide. Conclusions: The findings indicated that the IC kits could be used as an alternative method for direct detection of norovirus in clinical specimens covering a wide range of norovirus genotypes circulating in Japan.
ISSN: 14336510
Appears in Collections:CMUL: Journal Articles

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