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Title: | Comparison of capillary electrophoregram among heterozygous Hb Hope, Hb Hope/α-thalassemia-1 SEA type deletion and Hb Hope/β0- thalassemia |
Authors: | Sakorn Pornprasert Sitthichai Panyasai Kanyakan Kongthai |
Authors: | Sakorn Pornprasert Sitthichai Panyasai Kanyakan Kongthai |
Keywords: | Biochemistry, Genetics and Molecular Biology;Medicine |
Issue Date: | 1-Sep-2012 |
Abstract: | Background: Hemoglobin (Hb) A2is artifactually elevated in cases of heterozygous Hb Hope when measured by capillary electrophoresis (CE). However, there is no report of HbA2levels and capillary electrophoregrams for associa-tions of heterozygote of Hb Hope with α-thalassemia nor β-thalassemia. Methods: Levels of HbA0, HbA2and Hb Hope in 16 heterozygous Hb Hope, 3 Hb Hope/a-thalassemia-1 SEA type deletion and 2 Hb Hope/β0- thalassemia were measured by CE. Electrophoregram and the levels of those were compared within these three groups. Results: Artifactually elevated HbA2levels (≥4%) were found in both groups of heterozygous Hb Hope and Hb Hope/α-thalassemia-1 SEA type deletion. Manual corrections were performed by adjusting baselines, and results showed that means of HbA2in both groups decreased from 4.47 % and 4.03 % to 1.93 % and 1.77 %, respectively. The highest levels of HbA2and Hb Hope were observed in samples with Hb Hope/α0-thalassemia. Moreover, HbA0was not observe in these cases. Conclusions: The elevation of HbA2in patients with heterozygous Hb Hope and with Hb Hope/α-thalassemia-1 SEA type deletion measured by CE leads to incorrect β-thalassemia trait diagnosis. However, using CE electrophoregram together with levels of HbA0, HbA2and Hb Hope would be a more accurate and precise method for diagnosis of Hb Hope/βdeg;-thalassemia. © 2012 by Walter de Gruyter. Berlin. Boston. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84867806942&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/51362 |
ISSN: | 14374331 14346621 |
Appears in Collections: | CMUL: Journal Articles |
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