Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/51290
Title: Preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin
Authors: Surat Hongsibsong
Jiraprapa Wipasa
Mookda Pattarawarapan
Prasak Thavornyutikarn
Somporn Chantara
Tippawan Prapamontol
Keywords: Agricultural and Biological Sciences
Issue Date: 21-Mar-2012
Abstract: This study aimed at developing a method for the preparation of hapten and immunogen for producing polyclonal antibody specific to cypermethrin for the determination of insecticide residues in contaminated fruits and vegetables. 3-(2, 2-dichlorovinyl)-2, 2-dimethyl - (1-cyclopane) carboxylic acid (DCCA) was used as a spacer arm of hapten preparation. DCCA was linked to beta alanine methyl ester and conjugated with bovine serum albumin (BSA) as an immunogen and with oval albumin (OVA) as a coating antigen. BALB/c mice were immunized with immunogen. After three doses, sera from each mouse were collected and used to develop an ELISA. 0.05% Tween20/PBS with 20% methanol was used as diluents. The obtained polyclonal antibody (pAb) gave IC50 to cypermethrin at 5.2 μg/well. The lowest quantification limit of detection of the ELISA was 0.18 μg/well at 85% B/B0 and the detection limit was 0.039 μg/well at 90% B/B0. The antibody will be applicable for developing ELISA for screening of cypermethrin residue in vegetable and fruit samples and enable to save cost of currently used analytical procedures. © 2012 Friends Science Publishers.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84858314833&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/51290
ISSN: 18149596
15608530
Appears in Collections:CMUL: Journal Articles

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