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|Title:||Genotypic linkages of gene segments of rotaviruses circulating in pediatric patients with acute gastroenteritis in Thailand|
|Keywords:||Agricultural and Biological Sciences|
Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
|Abstract:||Rotavirus is a major cause of morbidity and mortality of infants and young children with diarrhea throughout the world. In Thailand, extensive studies of rotavirus infections have been reported continually and rotavirus diarrhea remains a common illness. To monitor the epidemiological situation of rotavirus in Chiang Mai, Thailand, surveillance of rotavirus circulating in pediatric patients was conducted. A total of 160 fecal specimens collected from children hospitalized with diarrhea were tested for rotaviruses groups A, B, and C by RT-PCR and their genotypes were identified by multiplex PCR and nucleotide sequencing. Group A rotavirus was detected at 29.4% but none of group B and C was found in this study. Molecular characterizations of G- and P-genotypes revealed three different G-P combinations, G1P was the most predominant genotype with the prevalence of 72.3% followed by G2P at 19.2%, and G3P at 8.5%. Phylogenetic analyses of VP7 and VP4 genes of the representative strains detected in the present study, G1, G2, G3, and P and P, respectively, revealed that G1 belonged to G1-Ic and G1-II, G2 belonged to G2-II, and G3 belonged to G3-III-S4 lineages while P and P were identified as P-V and P-III lineages. Analyses of VP6, NSP4, and NSP5 genes demonstrated that these representative strains belonged to genotypes I1 and I2, E1 and E2, and H1 and H2, respectively. Analyzing the association of G- and P-genotypes with I, E, H genotypes revealed unique patterns of genotypic linkage. The G1P and G3P were intimately linked with I1, E1, H1 genotypes and displayed the genetic features of G1-P-I1-E1-H1 and G3-P-I1-E1-H1, respectively, while G2P was closely linked to I2, E2, H2 genotypes and showed the genetic pattern of G2-P-I2-E2-H2. This study provides epidemiological information and insight into the genetic background of rotaviruses circulating in pediatric patients in Chiang Mai, Thailand. © 2012 Elsevier B.V.|
|Appears in Collections:||CMUL: Journal Articles|
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