Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/49818
Title: Sequential injection Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme
Authors: Kraingkrai Ponhong
Supaporn Kradtap Hartwell
Kate Grudpan
Keywords: Chemistry
Issue Date: 15-Jul-2011
Abstract: A sequential injection-Lab-at-valve (SI-LAV) segmented flow system for kinetic study of an enzyme was developed. Air segments were introduced for separation of enzyme and substrate zones and separation of the stacked zones from the carrier solution which ensure the measurement of the initial rate and minimize the dilution/dispersion effect. The open- ended mixing chamber makes it possible to use air segments in the flow system without the need for additional air segment discarding steps. The enzyme horseradish peroxidase (HRP) kinetic parameters based on initial rate was used as a model study. The operation of the system is virtually the same as that of the conventional batch-wise process. The kinetic parameters (i.e. Kmand Vmax) of HRP obtained using the proposed system agree well with those obtained using the batch-wise process as well. The proposed system offers additional benefits of volume down scaling, improved rapidity and automatic features that does not require a skillful operator. © 2011 Elsevier B.V. All rights reserved.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=79958102298&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/49818
ISSN: 00399140
Appears in Collections:CMUL: Journal Articles

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