Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/39902
Title: อาหารอย่างง่ายสำหรับการเพาะเลี้ยงโปรโตคอร์มกล้วยไม้เอื้องพร้าว
Other Titles: Simple Media for Tissue Culture of Phaiustaius tankerilleae protocorm
Authors: ผศ.ดร.ศรีสุลักษณ์ ธีรานุพัฒนา
นารถกาญจน์ ลภิรัตนากูล
Keywords: กล้วยไม้
กล้วยไม้เอื้องพร้าว
Issue Date: Jul-2557
Publisher: เชียงใหม่ : บัณฑิตวิทยาลัย มหาวิทยาลัยเชียงใหม่
Abstract: The purpose of this reseach was to study the simple culture media for Phaiustaius tankerilleae protocorm and to produce an instruction media of plant tissue culture for classroom. Phaiustaius tankerilleae's seeds were cultured on the VW (Vacin and Went) media for 4 weeks until the protocorms were developed. The protocorms were transferred to four simple culture media: OD (Oradee Sahavacharin), TisTR (Thailand Institube of Scientific and Technological Research), NK (Nattakan), and VW and incubated for 7 weeks. The results showed that the mean diameter of the protocorms were insiginficantly different (p<0.05). Moreover, the shoots were induced their at 100 % with these four media. The best results were obtained using VW and NK formula only could be measured that the average length of shoots were 18.00 and 17.08 mm respectively which were insiginficantly different (p<0.05). Besides, an instruction medium of plant tissue culture which contained lab direction and VDO demonstation was used in the classroom. The knowledge reqarding plant tissue culture was increased in students who used this media as soon by the increasing of pre-test score from 2.68 to 13.77 in post-test score. In addition, it the students were satisfied with thir teaching medium at the hightest level.
URI: http://cmuir.cmu.ac.th/jspui/handle/6653943832/39902
Appears in Collections:SCIENCE: Independent Study (IS)

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APPENDIX.pdfAPPENDIX1.33 MBAdobe PDFView/Open    Request a copy
CHAPTER 1.pdfCHAPTER 1166.04 kBAdobe PDFView/Open    Request a copy
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CHAPTER 5.pdfCHAPTER 5290.91 kBAdobe PDFView/Open    Request a copy
CHAPTER 6.pdfCHAPTER 6192.33 kBAdobe PDFView/Open    Request a copy
CONTENT.pdfCONTENT164.71 kBAdobe PDFView/Open    Request a copy
COVER.pdfCOVER472.25 kBAdobe PDFView/Open    Request a copy
REFERENCE.pdfREFERENCE280.92 kBAdobe PDFView/Open    Request a copy


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