การวิเคราะห์ผลการตรวจสารก่อภูมิต้านทานจำเพาะต่อมลูกหมากโดยใช้ชุดตรวจสำเร็จรูปเปรียบเทียบกับผลตรวจแอซิดฟอสฟาเทสในน้ำสกัดสำลีซับช่องคลอดที่ตรวจพบและไม่พบตัวอสุจิ

Abstract

Sexual offences, particularly in cases of sexual abuse, are crimes that occur frequently. Criminalistics requires scientific evidence. This study was the detection of prostate specific antigen using test kit compared to enzyme acid phosphatase test based on biochemical methods. Sixty vaginal swabs were collected 30 were semen detected and 30 were semen undetected samples. The samples were then eluted from the swab by soaking in distilled water. The fluid part was determined for the enzyme acid phosphatase and for the prostate specific antigen by using test kit. The sludge was collected and spreaded on glass slides and let dried at room temperature before staining by Oppitz method. The results revealed that semen detected samples were all positive when tested by test kit and the enzyme acid phosphatase test. The semen undetected samples showed that eight samples of the test kit results were positive, and ten samples of the enzyme acid phosphatase test were positive. However, it was not statistically different. In some cases, when semen was not detected, it did not mean that there was no semen. One case showed negative result on the enzyme acid phosphatase test but the test kit result was positive. When the case’s historical background was checked, it revealed that the case was tested within 24 hours. This could be assumed that the semen found in the victim revealed that the test kit was accurate. This could help reduce the error in the case where the suspect was barren and three cases showed positive results of the enzyme acid phosphatase test. In contrast, the test kit showed negative results. When the historical background was checked, it revealed that the enzyme acid phosphatase test showed positive results at the latter period of test time. This could be false positive results. Therefore, it was concluded that test kits could be appropriately used in routine casework for rape investigation.