ผลของไคโตซานและไซเปอร์เมทรินต่อการเปลี่ยนแปลงรูปร่างและจุลกายวิภาคของไตในตัวอ่อนกบนา (Hoplobatrachus rugulosus)

Abstract

The declines of amphibian populations have been reported, in part, to be associated with the toxicity of pesticides contaminated in natural water resources. The present study aim to investigate the effects of chitosan and cypermethrin on metamorphosis and histological alteration of kidney in Hoplobatrachus rugulosus larvae and to produce the laboratory guideline for science class of secondary school. In this regard, The5-day-old larvae were either exposed to 0.2 ppm, 1.0 ppm and 5.0 ppm of chitosan or/of cypermethrin for 30 days. During the experiment, mortality, growth, and metamorphosis were recorded. The kidneys were sampled for histological examination at the end of experiment. The results revealed that chitosan and cypermethrin could significantly decrease the survivorship of the tadpole (p≤0.05). However, the mortality increased with the higher concentration of cypermethrin but not chitosan. The time to metamorphosis was decreased with increasing of cypermethrin but increased with higher concentration of chitosan. On the contrary, these two chemicals had no effect on the change of body length. The histopathological finding was also found in both of cypermethrin and chitosan exposure groups. The shrinking of glomerulus was observed together with amorphous substances inBowman’s Capsule Space. Moreover, the laboratory guideline was created for the demonstration.The knowledge obtained from this study was demonstrated to the student to increase the understanding of agricultural chemical toxicity.