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Title: Development of glucose measurement system for automatic tissue engineering cell culture
Other Titles: การพัฒนาระบบตรวจวัดกลูโคสเพื่อการเพาะเลี้ยงเซลล์อัตโนมัติ สำหรับงานวิศวกรรมเนื้อเยี่อ
Authors: Chanyanut Wongsa
Authors: Pathinan Paengnakorn
Kate Grudpan
Chanyanut Wongsa
Issue Date: 9-Jun-2023
Publisher: Chiang Mai : Graduate School, Chiang Mai University
Abstract: The study presents a novel system for monitoring glucose in cell culture, incorporating the use of gold nanoparticles, single-walled carbon nanotubes, glucose oxidase enzyme, and chitosan solution (AuNP-SWCNT-GOD-CS) modified on a commercial carbon screen-printed electrode (SPE). This system is integrated with a sequential injection analysis (SIA) system, which includes a flow cell, syringe pump, selective valve, mixing channel, holding coil, and a six-well plate for cell inoculation. The system allows for the operation of six separate cell culture units under different conditions within the same environment. The modified glucose measurement system offers automated in-line sampling and sample dilution through a mixing unit. This ensures rapid analysis and reduces the risk of contamination. Additionally, the utilization of 3D-printed microfluidic components, a mixing channel, and a flow cell helps streamline operations and minimize sample volume. The developed system can detect glucose linearly up to a concentration of 3.8 mM in cell culture media. In cases where higher glucose concentrations are present, in-line sample dilution can be implemented. The effectiveness of the developed glucose measurement system was verified through successful testing with mouse fibroblast (L929) cell culture, producing results that aligned with those obtained using the traditional colorimetric method. This research showcases significant potential for further advancement and application in the field of biomedical research, particularly for in vitro cell-based experiments.
Appears in Collections:BMEI: Theses

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