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Title: | Development of multiplex PCR for identification of species of the lindesayi complex (Diptera: Culicidae) in Bhutan and China |
Other Titles: | การพัฒนามัลติเพล็กซ์ PCR เพื่อการจำแนกชนิดของ Lindesayi Complex (Diptera: Culicidae) ในประเทศภูฏานและประเทศจีน |
Authors: | Zhou, Li |
Authors: | Pradya Somboon Atiporn Saeung Jassada Saingamsook Zhou, Li |
Issue Date: | Jul-2023 |
Publisher: | Chiang Mai : Graduate School, Chiang Mai University |
Abstract: | Five sibling species of the Lindesayi Complex of the genus Anopheles have been discovered in Bhutan: An. druki Somboon, Namgay & Harbach, An. himalayensis Somboon, Namgay & Harbach, An. lindesayi Giles, An. lindesayi species B and An. thimphuensis Somboon, Namgay & Harbach. The species are morphologically similar in the adult and/or immature stages. This study aimed to develop a multiplex PCR assay to identify the five species. Allele-specific primers were designed for specific nucleotide segments of ITS2 sequences previously reported for each species. The assay provided products of 183 bp for An. druki, 338 bp for An. himalayensis, 126 bp for An. lindesayi, 290 bp for An. lindesayi species B and 370 bp for An. thimphuensis. Use of the assay produced consistent results. The assay is relatively inexpensive, enables the rapid identification of a large number of specimens and will foster further studies of the Lindesayi Complex. |
URI: | http://cmuir.cmu.ac.th/jspui/handle/6653943832/78609 |
Appears in Collections: | MED: Theses |
Files in This Item:
File | Description | Size | Format | |
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Thesis Ms.Li Zhou 630755804.pdf | 1.13 MB | Adobe PDF | View/Open Request a copy |
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