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dc.contributor.authorWorraprat Chaisuwanen_US
dc.contributor.authorApisit Manassaen_US
dc.contributor.authorYuthana Phimolsiripolen_US
dc.contributor.authorKittisak Jantanasakulwongen_US
dc.contributor.authorThanongsak Chaiyasoen_US
dc.contributor.authorWasu Pathom-Areeen_US
dc.contributor.authorSangguan Youen_US
dc.contributor.authorPhisit Seesuriyachanen_US
dc.description.abstractFructooligosaccharides (FOS) are considered prebiotics and have been widely used in various food industries as additives. Ultrasonication has been widely used to enhance food processes; however, there are few reports on ultrasound-assisted FOS synthesis. In the present study, FOS were produced from brown sugar using ultrasonication combined with microbubbles, and the production was optimised using a Box-Behnken experimental design. Here we showed that a combination of ultrasonication and microbubbles could boost the enzyme activity by 366%, and the reaction time was shortened by 60%. The reaction time was a significant variable affecting the FOS production. The optimum conditions were 5 min 45 s of ultrasonication and 7 min 19 s of microbubbles with a reaction time of 5 h 40 min. The maximum enzyme activity and total FOS yield were 102.51 ± 4.69 U·mL−1 and 494.89 ± 19.98 mg·g−1 substrate, respectively. In an enlarged production scale up to 5 L, FOS yields were slightly decreased, but the reaction time was decreased to 4 h. Hence, this technique offers a simple and useful tool for enhancing enzyme activity and reducing reaction time. We have developed a pilot technique as a convenient starting point for enhancing enzyme activity of oligosaccharide production from brown sugar.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectHealth Professionsen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectSocial Sciencesen_US
dc.titleIntegrated ultrasonication and microbubble-assisted enzymatic synthesis of fructooligosaccharides from brown sugaren_US
article.volume9en_US National Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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