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dc.contributor.authorMarninphan Thongkhamen_US
dc.contributor.authorWannaluk Thawornen_US
dc.contributor.authorWiwat Pattanawongen_US
dc.contributor.authorSorn Teepatimakornen_US
dc.contributor.authorSupamit Mekchayen_US
dc.contributor.authorKorawan Sringarmen_US
dc.description.abstractThis study compared the quality parameters of bull semen sexed using an immunological method with those of conventional semen by imaging flow cytometry and applied this semen in dairy farm trials. Semen samples were collected from ten ejaculates from five bulls. Each sample was divided into two treatments: conventional semen (CON) and semen sexed using monoclonal male-specific antibodies combined with the complement system for cytotoxicity reaction (IC-sexed). After obtaining frozen–thawed semen, we used imaging flow cytometry to assess acrosome integrity, sperm sex ratio and viability. Sperm morphology was evaluated using eosin–nigrosin staining. The percentage acrosome integrity did not differ between IC-sexed and CON semen (P = 0.313). The sperm sex ratio showed that the percentage of live X-chromosome-bearing sperm was higher than that of live Y-chromosome-bearing sperm in IC-sexed semen (P = 0.001). IC-sexed semen showed a higher percentage of head and tail defects than did CON semen (P = 0.019). In field trials, 585 cows were subjected randomly to AI with CON or IC-sexed semen. The pregnancy rate of the IC-sexed group did not differ from that of the CON group (P = 0.535). However, IC-sexed semen produced a significantly higher percentage of female calves than did CON semen (P = 0.031). Thus, immunological sexing did not adversely affect the acrosome integrity of sperm. Furthermore, a female calf birth rate of over 74 % can potentially be achieved using IC-sexed semen. These findings could help farmers to replace heifers in their herds.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleSpermatological parameters of immunologically sexed bull semen assessed by imaging flow cytometry, and dairy farm trialen_US
article.title.sourcetitleReproductive Biologyen_US
article.volume21en_US Universityen_US Ministry of Agriculture and Cooperativesen_US of Higher Education, Science, Research and Innovationen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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