Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/74996
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dc.contributor.authorSaeed Hajirezaeeen_US
dc.contributor.authorAmirreza Abed-Elmdousten_US
dc.contributor.authorNatalia Alekhinaen_US
dc.contributor.authorSupat Chupraditen_US
dc.contributor.authorYasser Fakri Mustafaen_US
dc.date.accessioned2022-10-16T06:56:08Z-
dc.date.available2022-10-16T06:56:08Z-
dc.date.issued2021-12-01en_US
dc.identifier.issn18780407en_US
dc.identifier.issn1744117Xen_US
dc.identifier.other2-s2.0-85116078126en_US
dc.identifier.other10.1016/j.cbd.2021.100917en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85116078126&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/74996-
dc.description.abstractA metabolomics study was conducted to investigate the molecular bases of oocyte over-ripening in common carp, Cyprinus carpio from a metabolic point of view. The ovulation was induced in fish brooders by intramuscular injection of pituitary extract and oocytes were collected four times post-ovulation with 30 min intervals. A set of 32 metabolites were identified on the NMR spectra of the oocytes, which mainly included energy-linked metabolites, amino acids, methylated metabolites and citric acid cycle (TCA) intermediates. PCA and PLS-DA models clearly separated the post ovulations times, indicating the effects of post-ovulation time on oocyte metabolome content. Based on the loading plot outputs, 15 metabolites including tryptophan, cysteine, AMP, tyrosine, valine, creatine phosphate (PCr), ATP, leucine, inosine, malate, acetate, TMAO, glucose, fumarate and lysine had more effects on the separation of post ovulation times. According to the results of metabolite profiling, the concentrations of glutamine, alanine, tryptophan, lysine and cysteine mostly significantly (P < 0.01) increased at 90 and 120 min post-ovulation. The concentrations of PCr, ATP, inosine and guanosine were relatively stable until 60 min post-ovulation, while significantly (P < 0.01) decreased at 90 and 120 min post ovulation. The TCA metabolites succinate, malate and fumarate significantly (P < 0.01) elevated at 90 and 120 min post-ovulation. AMP concentrations remained relatively unchanged until 30 min and then progressively decreased with time post ovulation (P < 0.01). The concentrations of lactate showed significant elevations at 90 and 120 min post ovulation (P < 0.01). In conclusion, the energetic potentials of the oocytes reduced with time post ovulation. There were apparent elevations in the concentrations of free amino acids, which may be associated with the onset of proteolytic activities in the post ovulatory oocytes. In addition, we found some changes in the apoptotic-related metabolites, which may support the results of previous studies regarding the oxidative stress and following apoptosis in post ovulatory oocytes of fish.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleMetabolite profiling of the post-ovulatory oocytes of the common carp, Cyprinus carpio: A <sup>1</sup>H NMR-based metabolomics approachen_US
dc.typeJournalen_US
article.title.sourcetitleComparative Biochemistry and Physiology - Part D: Genomics and Proteomicsen_US
article.volume40en_US
article.stream.affiliationsUniversity of Jiroften_US
article.stream.affiliationsUniversity of Mosulen_US
article.stream.affiliationsUniversity of Tehranen_US
article.stream.affiliationsSechenov First Moscow State Medical Universityen_US
article.stream.affiliationsChiang Mai Universityen_US
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