Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSayamon Hongjaiseeen_US
dc.contributor.authorYosita Jabjainaien_US
dc.contributor.authorSuthasinee Sakseten_US
dc.contributor.authorKanya Preechasuthen_US
dc.contributor.authorNicole Ngo-Giang-huongen_US
dc.contributor.authorWoottichai Khamduangen_US
dc.description.abstractNucleic acid extraction from biological samples is an important step for hepatitis C virus (HCV) diagnosis. However, such extractions are mostly based on silica-based column methodologies, which may limit their application for on-site diagnosis. A simple, rapid, and field-deployable method for RNA extraction is still needed. In this study, we evaluated the efficacy of four simple RNA extraction methods for the detection of HCV in plasma samples: a silica-membrane-based method, a magnetic-beads-based method, boiling with diethyl pyrocarbonate (DEPC)-treated distilled water, and using a commercial lysis buffer. HCV RNA was detected using both real-time reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP). Using real-time RT-PCR, extracted RNA from the silica-membrane-based and magnetic-beads-based methods had a 100% detection rate for RNA extraction from plasma. Using RT-LAMP, extracted RNA from the silica-membrane-based method showed a 66% detection rate, while the magnetic-beads-based method had a 62% detection rate. In summary, magnetic-beads-based extraction can be used as an alternative RNA extraction method for on-site HCV detection. Boiling with DEPC-treated distilled water was not appropriate for low HCV load samples, and boiling with a lysis buffer was not recommended.en_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleComparison of Simple RNA Extraction Methods for Molecular Diagnosis of Hepatitis C Virus in Plasmaen_US
article.volume12en_US of Phayaoen_USé de Montpellieren_US Mai Universityen_US Medical Sciences (AMS)-PHPT Research Collaborationen_US
Appears in Collections:CMUL: Journal Articles

Files in This Item:
There are no files associated with this item.

Items in CMUIR are protected by copyright, with all rights reserved, unless otherwise indicated.