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|Title:||Anti-Cancer Compound Screening and Isolation: Coscinium fenestratum Tinospora crispa and Tinospora cordifolia|
|Publisher:||Faculty of Science, Chiang Mai University|
|Citation:||Chiang Mai Journal of Science 36, 3 (September 2010), 476-488|
|Abstract:||The isolations of active anti-cancer compounds from Coscinium fenestratum, Tinospora crispa and Tinospora cordifolia are rarely reported. In this study, the isolation of anti-cancer compound from these herbs was performed. Cytotoxic activity of the water and methanol extracts of these herbs were evaluated. The methanol extract of C. fenestratum showed the most cytotoxic activity, and contained a major compound of berberine with 3.68%. Cytotoxic screening of berberine against ten cancer cell lines and one normal cell (PMBC) showed the most cytotoxic activity against HL-60 leukemia cells with an IC50 of 1.41+/- 0.7 μg/mL. The selective index (SI) value of berberine against HL-60 cells and PBMC cells were 0.142, indicating the selective cytotoxicity to cancer cells. Interestingly, berberine uptake by cancer cell correlated with cytotoxic activity. In addition, the apoptosis of HL-60 cells against berberine were detected by DAPI staining, and quantitated by Annexin V-FITC staining. Berberine, the effective compound from C. fenestratum, appears to possess anticancer potential.|
|Description:||The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.|
|Appears in Collections:||CMUL: Journal Articles|
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