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dc.contributor.authorJirapak Ruttanapattanakulen_US
dc.contributor.authorNitwara Wikanen_US
dc.contributor.authorSiriporn Okonogien_US
dc.contributor.authorMingkwan Na Takuathungen_US
dc.contributor.authorPensiri Buacheenen_US
dc.contributor.authorPornsiri Pitchakarnen_US
dc.contributor.authorSaranyapin Potikanonden_US
dc.contributor.authorWutigri Nimlamoolen_US
dc.description.abstract© 2020 The Authors Boesenbergia rotunda (BR) has long been used as tradition medicine. For its pharmacological effects on wound healing, previous studies in an animal model provided convincing results that the ethanolic extract from the rhizome of this plant can stimulate wound healing. However, the mechanism about how this plant promotes wound healing at the molecular level has not been elucidated. As a step towards the development of wound healing agents, our current study utilized a human keratinocyte cell line (HaCaT) as an in vitro model to define the potential molecular mechanisms of BR extract in enhancing wound-healing. Our HPLC results showed that BR extract contained kaempferol as one of its potential compounds. The extract strongly promoted wound healing of HaCaT cell monolayer. This effect was eventually defined to be regulated through the ability of BR extract to induce cell proliferation. At the signaling level, we discovered that BR extract rapidly activated ERK1/2 and Akt phosphorylation upon the addition of the extract. Additionally, our experiments where specific inhibitors of MEK (U0126) and PI3K (LY294002) were utilized verified that BR enhanced cell proliferation and wound healing through stimulating the MAPK and PI3K/Akt signal transduction pathways. Moreover, direct inhibition of keratinocyte DNA synthesis by mitomycin C (MMC) could completely block the proliferative effects of BR extract. Nevertheless, data from Transwell migration assay revealed that BR extract did not promote keratinocyte migration. Altogether, we provided more evidence that BR possesses its wound healing-promoting action through the activation of proliferation and survival pathways, and our study suggests that BR is an interesting candidate to be developed as a wound healing-promoting agent.en_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleBoesenbergia rotunda extract accelerates human keratinocyte proliferation through activating ERK1/2 and PI3K/Akt kinasesen_US
article.title.sourcetitleBiomedicine and Pharmacotherapyen_US
article.volume133en_US Universityen_US Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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