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dc.contributor.authorSayamon Hongjaiseeen_US
dc.contributor.authorNatteewan Doungjindaen_US
dc.contributor.authorWoottichai Khamduangen_US
dc.contributor.authorTanawan Samleerat Carrawayen_US
dc.contributor.authorJiraprapa Wipasaen_US
dc.contributor.authorJose D. Debesen_US
dc.contributor.authorKhuanchai Supparatpinyoen_US
dc.date.accessioned2021-01-27T04:18:20Z-
dc.date.available2021-01-27T04:18:20Z-
dc.date.issued2021-01-01en_US
dc.identifier.issn18783511en_US
dc.identifier.issn12019712en_US
dc.identifier.other2-s2.0-85097065349en_US
dc.identifier.other10.1016/j.ijid.2020.10.082en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85097065349&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/71944-
dc.description.abstract© 2020 The Author(s) Objectives: The aim was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of hepatitis C virus (HCV) in a single closed tube. Methods: Plasma samples were collected from 200 HCV-infected patients. HCV-RNA was detected by one-step RT-LAMP processed at 65 °C for 60 min. The amplified products were detected by hydroxynaphthol blue (HNB)-dependent visual method and gel electrophoresis. Specificity was tested against other viruses. Sensitivity was determined using serial dilutions of extracted RNA. Results: The RT-LAMP assay detected 97.5% of HCV-RNA genotype 1, 91.1% of genotype 3, and 100% of genotype 6. The color change was evidenced with the naked eye. The assay demonstrated a clinical sensitivity of 95.5% and specificity of 100%, as well as no cross-reactivity with other viruses (i.e., hepatitis B virus, HIV). The limit of detection was as low as 10 ng per reaction for HCV genotypes 1a and 6, while it was 100 ng for genotype 3a. The assay showed a 100% detection threshold at a viral load of 5.00 log10 IU/mL in the clinical samples tested. Conclusions: This study demonstrated the use of an RT-LAMP assay for the detection of HCV in a simple, rapid, and cost-effective manner, which will be useful in resource-limited settings to allow the identification of individuals in need of HCV treatment.en_US
dc.subjectMedicineen_US
dc.titleRapid visual detection of hepatitis C virus using a reverse transcription loop-mediated isothermal amplification assayen_US
dc.typeJournalen_US
article.title.sourcetitleInternational Journal of Infectious Diseasesen_US
article.volume102en_US
article.stream.affiliationsUniversity of Minnesota Twin Citiesen_US
article.stream.affiliationsChiang Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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