Please use this identifier to cite or link to this item: http://cmuir.cmu.ac.th/jspui/handle/6653943832/71816
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dc.contributor.authorYuta Kanaien_US
dc.contributor.authorMisa Onishien_US
dc.contributor.authorTakahiro Kawagishien_US
dc.contributor.authorPimfhun Pannachaen_US
dc.contributor.authorJeffery A. Nurdinen_US
dc.contributor.authorRyotaro Noudaen_US
dc.contributor.authorMoeko Yamasakien_US
dc.contributor.authorTina Lusianyen_US
dc.contributor.authorPattara Khamrinen_US
dc.contributor.authorShoko Okitsuen_US
dc.contributor.authorSatoshi Hayakawaen_US
dc.contributor.authorHirotaka Ebinaen_US
dc.contributor.authorHiroshi Ushijimaen_US
dc.contributor.authorTakeshi Kobayashien_US
dc.date.accessioned2021-01-27T04:16:17Z-
dc.date.available2021-01-27T04:16:17Z-
dc.date.issued2021-01-01en_US
dc.identifier.issn10985514en_US
dc.identifier.issn0022538Xen_US
dc.identifier.other2-s2.0-85098662886en_US
dc.identifier.other10.1128/JVI.01374-20en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85098662886&origin=inwarden_US
dc.identifier.urihttp://cmuir.cmu.ac.th/jspui/handle/6653943832/71816-
dc.description.abstractCopyright © 2020 American Society for Microbiology. All Rights Reserved. Species A rotaviruses (RVs) are a leading cause of severe acute gastroenteritis in infants and children younger than 5 years. Currently available RV vaccines were adapted from wild-type RV strains by serial passage of cultured cells or by reassortment between human and animal RV strains. These traditional methods require large-scale screening and genotyping to obtain vaccine candidates. Reverse genetics is a tractable, rapid, and reproducible approach to generating recombinant RV vaccine candidates carrying any VP4 and VP7 genes that provide selected antigenicity. Here, we developed a vaccine platform by generating recombinant RVs carrying VP4 (P[4] and P[8]), VP7 (G1, G2, G3, G8, and G9), and/or VP6 genes cloned from human RV clinical samples using the simian RV SA11 strain (G3P[2]) as a backbone. Neutralization assays using monoclonal antibodies and murine antisera revealed that recombinant VP4 and VP7 monoreassortant viruses exhibited altered antigenicity. However, replication of VP4 monoreassortant viruses was severely impaired. Generation of recombinant RVs harboring a chimeric VP4 protein for SA11 and human RV gene components revealed that the VP8* fragment was responsible for efficient infectivity of recombinant RVs. Although this system must be improved because the yield of vaccine viruses directly affects vaccine manufacturing costs, reverse genetics requires less time than traditional methods and enables rapid production of safe and effective vaccine candidates. IMPORTANCE Although vaccines have reduced global RV-associated hospitalization and mortality over the past decade, the multisegmented genome of RVs allows reassortment of VP4 and VP7 genes from different RV species and strains. The evolutionary dynamics of novel RV genotypes and their constellations have led to great genomic and antigenic diversity. The reverse genetics system is a powerful tool for manipulating RV genes, thereby controlling viral antigenicity, growth capacity, and pathogenicity. Here, we generated recombinant simian RVs (strain SA11) carrying heterologous VP4 and VP7 genes cloned from clinical isolates and showed that VP4- or VP7-substituted chimeric viruses can be used for antigenic characterization of RV outer capsid proteins and as improved seed viruses for vaccine production.en_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleReverse genetics approach for developing rotavirus vaccine candidates carrying VP4 and VP7 genes cloned from clinical isolates of human rotavirusen_US
dc.typeJournalen_US
article.title.sourcetitleJournal of Virologyen_US
article.volume95en_US
article.stream.affiliationsResearch Institute for Microbial Diseasesen_US
article.stream.affiliationsNihon University School of Medicineen_US
article.stream.affiliationsChiang Mai Universityen_US
Appears in Collections:CMUL: Journal Articles

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