Effect of SNPs in genes regulating cell cycle control, apoptosis and inflammation on lung cancer susceptibility of northern Thai population

Abstract

© 2020 Science Society of Thailand under Royal Patronage. All rights reserved. Northern Thai population has experienced a high incidence of lung cancer and greater rates of mortality over the past several decades. In this study, blood samples and personal information were collected from 91 non-small cell lung cancer patients and 84 cancer-free healthy unrelated volunteers living in northern Thailand. Eight functional SNPs, including cell cycle control (TP53 Pro72Arg, MDM2 T309G, CCND1 G723A, CDKN1A Ser31Arg), apoptosis (FASLG C-844T, FAS G-1378A) and inflammation (TGFB1 T-1347C, TGFB1 Pro10Leu) were genotyped by multiplex allele-specific polymerase chain reactions. Odds ratios (OR) with 95% confidence intervals (CI) were calculated using logistic regression after making adjustments for age, gender and the smoking status of all participating subjects. No significant association between a single SNP and lung cancer risk was observed. However, a combination of TGFB1-1347T and 10Pro allele carriers indicated a significantly increased risk for lung cancer (OR = 2.90, 95% CI = 1.01-8.4, P = 0.049) relative to wild-type genotypes. Among the participants, who had kitchens inside their homes, -1347T allele carriers had significant increase in lung cancer risk (OR = 5.9, 95% CI = 1.32-26.5, P = 0.020) when compared to the TGFB1-1347 C/C genotype carriers. In conclusion, we have observed a significant association between TGFB1-1347T allele and either TGFB1 10Pro allele or having kitchen inside the house on risk elevation of non-small cell lung cancer among the population of northern Thailand.