Effect of oral administration of chia (Salvia hispanica L.) seed extract on wound healing property in diabetic mice

Abstract

Chia, Salvia hispanica L., a plant containing lipid-antioxidant, has been shown to be beneficial for prevention of risk factors of type 2 diabetes. The objectives of this study were to evaluate oral chia seed extract on wound healing properties including wound contraction and histopathological examination in a diabetic wound model. C57BL/6J mice were fed with standard and high-fat diet for 27 weeks resulting in non-diabetic and diabetic mice respectively, then divided into 4 groups (n=6) as follows: normal control group, fed with standard diet; 4% chia seed extract group, fed with chia seed extract diet; glipizide group, fed with high-fat diet and glipizide; diabetic group; only fed with high-fat diet. The percentage of wound contraction, histopathological score and morphology were compared for evaluating wound healing properties. On day 12 post-wounding, a significant increase in the percentage of wound healing was found in the normal control, 4% chia seed extract and glipizide group as compared to diabetic group (p < 0.05, 99.45 ± 0.865, 98.99 ± 1.948, 99.06 ± 0.779 vs. 81.41 ± 10.759). Histopathological scores of normal control, 4% chia seed extract and Glipizide showed better healing than in the diabetic group (p < 0.05, 10.50 ± 0.837, 10.00 ± 0.632, 9.92 ± 1.625 vs. 3.83 ± 1.169). The results of histopathological morphology showed consistent results with histopathological scores, in which collagen, fibroblast, epithelialization and neovascularization were dominant in granulation tissue of better scores. It may be concluded that chia seed extract was better for diabetic wound healing in mice.