Prevalence and antibiotic resistance of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli in healthy dogs in Chiang Mai

Abstract

The aim of the present study was to investigate the prevalence and the antibiotic resistance of ESBL-producing Escherichia coli in healthy dogs in Chiang Mai. Fecal and oral biofilm samples were collected from eighty nine healthy dogs presented at Veterinary Teaching Hospital Chiang Mai University for received vaccination or health checking. All samples were inoculated on MacConkey agar plates containing 1ug/ml of cefotaxime and E. coli confirmation was performed by using MALDI TOF MS. ESBL producing E. coli confirmation was performed by combination disc test and the positive isolates were subjected to antibiotic susceptibility testing using 12 antibiotic discs by disc diffusion methods. The prevalence of ESBL producing E. coli of fecal samples was 25.84 %. On the other hand ESBL producing E. coli from oral biofilm samples was not detected. All ESBL producing E. coli isolates were highly antibiotic drug resistant, especially ampicillin, cefazolin, cephalexin and clindamycin (100% resistant). Furthermore, they had multi-drug resistance pattern. It is concluded that fecal ESBL producing E. coli can be found in healthy dogs and leads to the high antibiotic resistant rates. This information may increase awareness of animal-to-human or animal-to-animal transmission.